In April 2020, the International Zebrafish Society (IZFS) launched the IZFS Webinar Series. The series includes a variety of research and professional development topics. The content of the webinars is designed for researchers from every career level and to share the latest scientific findings, methods, technologies, and products to facilitate research. The format provides direct access to experts in the field in an interactive online setting.
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Generation and Application of Conditional Knock-In Alleles in Zebrafish
September 28, 2021
10:00 - 11:00 a.m. EDT
Despite the advent of genome editing approaches in zebrafish, eliminating gene function in a cell- and stage-specific remains challenging. In this webinar, I will present our efforts to implement the Cre/lox system in zebrafish for this purpose. I will describe optimized conditions for single step generation of lox-flanked knock-in alleles. I will also provide a framework for application of zebrafish conditional knock-in alleles with inducible Cre driver lines to investigate cell- and stage-specific genetic function, including common pitfalls and essential steps for technical validation.
Nathan Lawson, PhD
University of Massachusetts Medical School
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