In April 2020, the International Zebrafish Society (IZFS) launched the IZFS Webinar Series. The series includes a variety of research and professional development topics. The content of the webinars is designed for researchers from every career level and to share the latest scientific findings, methods, technologies, and products to facilitate research. The format provides direct access to experts in the field in an interactive online setting.
Webinar registration is free to all IZFS members. Non-members may participate in each webinar for a fee of $25.00 USD. To register for webinars, please click here. If you have never been a member and would like to join, please click here. If your membership has lapsed and you would like to renew to participate in the webinars, please click here.
Evo-Devo; Enhancers and Innate Immunity
Featuring - Alexa Burger, PhD and Serge Mostowy, PhD
March 21, 2024
9:00-10:00am EDT | 1:00-2:00pm GMT
Conserved distant enhancers regulate Brachyury/T/Tbxt expression in the vertebrate notochord
Alexa Burger, PhD
Assistant Research Professor
Section of Developmental Biology, Department of Pediatrics, School of Medicine
University of Colorado Anschutz Medical Campus
The cell type-specific expression of key transcription factors is central to development and disease. Brachyury/T/TBXT is a major transcription factor for gastrulation, tailbud patterning, and notochord formation; however, how its expression is controlled in the mammalian notochord has remained elusive. We identified three conserved Brachyury-controlling notochord enhancers, T3, C, and I, in the mammalian Brachyury/T/TBXT locus that are active in transgenic assays in zebrafish, axolotl, and mouse. Acting as Brachyury-responsive, auto-regulatory shadow enhancers, in cis deletion of all three enhancers in mouse abolishes Brachyury/T/Tbxt expression selectively in the notochord, causing specific trunk and neural tube defects without gastrulation or tailbud defects. The three Brachyury-driving notochord enhancers are conserved beyond mammals in the brachyury/tbxtb loci of fishes, dating their origin to the last common ancestor of jawed vertebrates. Our data define the vertebrate enhancers for Brachyury/T/TBXTB notochord expression through an auto-regulatory mechanism that conveys robustness and adaptability as ancient basis for axis development.
Use of zebrafish to study Shigella-phagocyte interactions
Serge Mostowy, PhD
Professor of Celluar Microbiology
London School of Hygiene and Topical Medicine
We discovered that host cells can prevent the actin-based motility of Shigella by compartmentalizing bacteria inside septin cages for targeting to autophagy. From this we hypothesized that understanding the role of septins, an enigmatic component of the cytoskeleton, will provide insights into bacterial infection and cellular immunity. We recently pioneered a ‘bottom up’ cellular microbiology approach to study cellular immunity and discovered a fundamental link between bacterial cell biology and the assembly of septin cages around Shigella. A major issue is now to fully decipher the underlying molecular and cellular events, and to validate these events analyzed in vitro during bacterial infection in vivo using relevant animal models. We developed zebrafish as an important model to study the cell biology of infection and therapeutic potential of targeting the cytoskeleton in vivo. Our findings should be of interest for both cell and infection biologists, and provide cutting edge platforms for studies at the single cell and whole animal level.
New webinar schedule! IZFS will have a webinar the third Thursday of each month!
|March 21, 2024
|April 18, 2024
|May 23, 2024
|Single cell phenotyping
|June 20, 2024
|Early embryonic development
For a complete list of our archived webinars, please click here
Current members may access the recorded webinars by logging into the "members-only" portal
This educational format provides a unique opportunity for potential sponsors to connect with the most influential group of fish researchers in a virtual setting. The content of the webinars is designed to attract researchers from every career level and to share the latest scientific findings, methods, technologies and products to facilitate their research work. With this virtual format, sponsors not only have exposure to attendees accessing the live webinars, but additional exposure and continued visibility as webinars are archived on the IZFS website and available to members to view on demand.
Please reach out to Alyssa Gluth at email@example.com with any questions.