Recordings are available to members through the "members-only" portal.
“For my Ally is the Force”: Engineering Sensors for in vivo Imaging of Mechanosensation
Featuring - Periklis (Laki) Pantazis, PhD and Konstantinos Kalyviotis, PhD
Dan Gorelick, PhD, Jessica Plavicki, PhD and Ben Lovely, PhD
July 17, 2023
Cellular mechanosensation and mechanotransduction are inherent abilities of cells to perceive and respond to extrinsic and intrinsic mechanical stimuli. In this webinar, we will discuss engineering efforts aimed at developing biosensors for mechanical interrogation of biological systems. We will guide everyone through the insights gained from our research in developing a Piezo1 activity sensor called GenEPi (Genetically Encoded Piezo1 indicator), and we will also suggest future engineering approaches to visualize the unseen forces within cells.
Dr Konstantinos Kalyviotis
Dr Periklis (Laki) Pantazis
The ins (absorption) and outs (excretion) of zebrafish toxicology studies
Featuring - Dan Gorelick, PhD, Jessica Plavicki, PhD and Ben Lovely, PhD
April 19, 2023
Zebrafish have long been a powerful model with which to study impact of environmental factors to development and disease. In this webinar, we will discuss approaches, controls and potential hurdles of environmental studies by discussing tips and tricks we have learned and developed in our research programs. Our goal is to provide an open dialogue of the “ins (absorption)” and “outs (excretion),” of toxicology/teratology studies.
|Dr. Dan Gorelick
Baylor College of Medicine
|Dr. Jessica Plavicki
|Dr. Ben Lovely
University of Louisville
The zebrafish epigenome: past, present, and beyond
Featuring - Ozren Bogdanovic, PhD & Ferenc Mueller, PhD
February 9, 2023
Dr. Ozren Bogdanovic will discuss: “Epigenome reprograming during zebrafish embryogenesis”
Dr. Ferenc Mueller will discuss: “How to exploit DANIO-CODE resources for gene regulation”
Vertebrate embryogenesis requires tight orchestration of spatiotemporal gene expression patterns, which is achieved through the coordinated action of transcription factors and genomic regulatory marks such as DNA methylation and histone tail modifications. The zebrafish model system has been instrumental for our understanding of how these marks participate in vertebrate embryogenesis. Systematic functional annotation of the zebrafish genome achieved by the DANIO-CODE consortium and by other functional annotation efforts led to creation of a central repository to store and process zebrafish developmental functional genomic data. These resources aid streamlined access to diverse zebrafish genomics and epigenomics datasets. The zebrafish user community is encouraged to exploit and to contribute to these resources.
|Dr. Ozren Bogdanovic
|Dr. Ferenc Mueller
University of Birmingham
Practical considerations and approaches to zebrafish transgenesis:
Featuring - Christian Mosimann, PhD and Kristen Kwan, PhD
May 24, 2022
Trangenesis to generate reporters, effectors, and modifier lines in zebrafish has become a standard method in the field. In this webinar, we will discuss practical considerations of transgene design, involved components, steps for quality control, and more. We will solicit questions from the community to address common problems with transgenesis.
Kristen Kwan, PhD,
Christian Mosimann, PhD,
Live Imaging and multi-color labelling strategies for tracking cells and circuits over time
Featuring - Fabienne Poulain, PhD and Elke Ober, PhD
May 3, 2022
Dr. Fabienne Poulain will discuss “It gets better with time: dynamic refinement of neural circuits in vivo”
Dr. Elke Ober will discuss "FRaeppli, a multispectral imaging toolbox for cell tracing and dense tissue analysis"
Drs. Fabienne Poulain and Elke Ober will present recently developed transgenic approaches to label and visualize individual cells or specific cell populations during organ and circuit formation and maturation in real time. Their studies focus on deciphering processes in dense tissues at high spatial and temporal resolution.
|Dr. Fabienne Poulain,
Dept of Biological Sciences
University of South Carolina
|Dr. Elke Ober,
NNF Center for Stem Cell Biology (DanStem)
University of Copenhagen, Denmark
Optogenetics: Approaches in Zebrafish
Featuring - Stephanie Woo, PhD and Clare Buckley, PhD
April 20, 2022
Stephanie Woo, Ph.D. will discuss "Optimizing a light-activated gene expression system for use in zebrafish"
Dr. Clare Buckley will discuss “Using optogenetics in zebrafish to understand neural tube development”
Optogenetics is a powerful emerging tool for studying biological processes, allowing high spatiotemporal control over gene expression and protein signalling. In this webinar, we will discuss the development of TAEL/C120, a light-activated inducible expression system that is specifically optimized for use in zebrafish. We will also discuss optimisation of Phytochrome optogenetics in zebrafish, which can be used to manipulate protein localisation and signalling. We will demonstrate different applications of these systems, methods for light delivery and other practical considerations.
|Stephanie Woo, PhD
University of California Merced
|Clare Buckley, PhD
University of Cambridge
Cambridge, London, UK
Fish, Water and Sustainability: Looking to the Future
March 17, 2022
10:00 - 11:00 a.m. EDT
As researchers using fish as a model system we are absolutely dependent on the amount and quality of the water we use, yet the world is facing a water crisis characterized by both water scarcity and pollution. In this webinar I will cover how we incorporate fish, water, and sustainability into our science outreach program for people of all ages. I will also discuss water use in zebrafish facilities highlighting the magnitude of inefficient water use, potential solutions, and a project we are initiating in our fish facility to recover water. https://cienciaaltiro.cl/en/proyects/water-the-root-of-life/
Kate Whitlock, PhD
Universidad de Valparaiso, Valparaiso, Chile
Generation and Application of Conditional Knock-In Alleles in Zebrafish
September 28, 2021
10:00 - 11:00 a.m. EDT
Despite the advent of genome editing approaches in zebrafish, eliminating gene function in a cell- and stage-specific remains challenging. In this webinar, I will present our efforts to implement the Cre/lox system in zebrafish for this purpose. I will describe optimized conditions for single step generation of lox-flanked knock-in alleles. I will also provide a framework for application of zebrafish conditional knock-in alleles with inducible Cre driver lines to investigate cell- and stage-specific genetic function, including common pitfalls and essential steps for technical validation.
Nathan Lawson, PhD
University of Massachusetts Medical School
New Developments in Vascular Biology
July 1, 2021
10:00 - 11:00 a.m. ET
Please join us for a pair of dynamic presentations highlighting innovative advances in our understanding of vascular biology in zebrafish.
Dr. Karina Yaniv will discuss "The Role of Endothelial Cell "Biography" on Vascular Diversity"
Dr. Arndt Siekmann will discuss "The Making and Shaping of Blood Vessels: Zebrafish Fins are Taking the Center Stage"
Karina Yaniv, PhD
Arndt Siekmann, PhD
Innovations in zebrafish chromatin analysis: CUT&RUN, CUT&TAG and ChIP methods
May 4, 2021
9:00 - 10:00 a.m. ET and
4:00 - 5:00 p.m. ET
Zebrafish have emerged as a powerful model for understanding chromatin changes in the context of embryonic development. This seminar will highlight recent advances in understanding chromatin regulation during early embryogenesis, and will discuss how new technologies of CUT&RUN and CUT&TAG can facilitate chromatin analysis.
Mary Goll, PhD
Patrick Murphy, PhD
Disease Modeling: Discovery and Modeling of Undiagnosed Human Diseases
April 6, 2021
11:00 am - 12:00 p.m. ET and
8:00 - 9:00 p.m. ET
There are more than 400 million people worldwide who suffer from undiagnosed diseases. Many of these diseases are extremely rare with as few as a single affected individual. Zebrafish play a central role in validation of unknown disease genes and elucidation of the underlying mechanisms of pathphysiology. In this webinar, I will discuss our work as part of the Undiagnosed Diseases Network.
Monte Westerfield, PhD
University of Oregon
Neuro-Journey: From Forming Neuronal Connectivity to Generating Behavior
March 25, 2021
11:00 a.m. - 12:00 p.m. ET
Join us for an exciting webinar featuring a pair of dispatches on cutting-edge neuroscience research in zebrafish, spanning topics from the establishment of neuronal connectivity to the execution of lateralized motor behavior!
Harold Burgess, PhD
Corinne Houart, PhD
February 17, 2021
12:00 - 1:00 p.m. ET
Katherine Brown, PhD
Cecilia Moens, PhD
Fred Hutchinson Cancer Research Center
Didier Stainier, PhD
Max Planck Institute
In this webinar, we plan to cover various aspects of manuscript submission and publication – from journal selection, manuscript and cover letter preparation and communications with the editor, through to revisions, rebuttals and finalisation of the article. We hope this will be an interactive session and encourage participants to submit questions in advance and contribute to the discussion.
Just Under the Surface: Leveraging Zebrafish to Understand the Interplay Between Evolution and Development
January 14, 2021
9:00 - 10:00 a.m. ET
6:00 - 7:00 p.m. ET
Matthew Harris, PhD
Harvard Medical School
Not only is zebrafish a powerful model to understand gene function in development, this fish provides a valuable window to understand how evolution has shaped development, and conversely, how development shapes evolution. This talk will discuss how genetic analysis in this singular representative species can support broader analysis of all fishes, including us.
Drug Discovery: Can Zebrafish Screens Really Lead to New Human Medicines?
December 10, 2020
10:00 - 11:00 a.m. ET
7:00 - 8:00 p.m. ET
Randy Peterson, PhD
University of Utah
As the only vertebrate model organism amenable to scalable screens, the zebrafish has long been touted as having the potential to deliver new drug candidates. In the past few years, drug candidates discovered in zebrafish have finally begun to reach human trials, suggesting that the potential may be turning to reality. This webinar will illustrate the arc of zebrafish drug discovery from academic lab to the clinic as well as highlight key challenges and emerging opportunities in zebrafish drug discovery.
November 17, 2020
9:00 - 10:00 a.m. ET
3:00 - 4:00 p.m. ET
Richard White, MD, PhD
Memorial Sloan Kettering Cancer Center
In this webinar, we will discuss how the intersection of developmental programs and the microenvironment drive melanoma progression and metastasis. Additionally, we will touch on new transgenic technologies in zebrafish, including Transgene Electroporation (TEAZ) and combinatorial methods.
October 20, 2020
12:00 - 2:00 p.m. ET
|Jan Huisken, PhD - Morgridge Institute for Research||Zebrafish Light Sheet Imaging With a Modular, Portable Microscope|
|Elizabeth Hillman, PhD - Columbia University||Broad Applications of SCAPE Microscopy for High-Speed 3D Imaging in Zebrafish Larvae
|Misha Ahrens, PhD - HHMI Janelia Research Campus||Imaging Neuronal and Glial Activity in Behaving Zebrafish|
|Kristen Kwan, PhD - The University of Utah||Computational Approaches to Dissecting Developmental Tissue Morphogenesis|
|Elisabeth Kugler, PhD - University College London||Quantifying the 3D Zebrafish Brain Vasculature in LSFM Data|
|Jonas Hartmann, PhD - University College London||Exploring Morphogenesis with Imaging and Data Science|
Moderator: Debbie Yelon, PhD - University of California San Diego
Imaging and image analysis are central components of many types of zebrafish research. The presentations in our imaging workshop will highlight a variety of innovative approaches for capturing biologically informative images and for performing insightful image analysis.
Single Cell Genomics
September 29, 2020
12:00 - 1:00 p.m. ET
Tatjana Sauka-Spengler, PhD
University of Oxford
How to Interview for a Zebrafish PI Position Workshop
August 27, 2020
9:00 - 10:30 a.m. ET
Phil Ingham, PhD Lila Solnica-Krezel, PhD
Phil Ingham, PhD - Nanyang Technological University
Bushra Raj, PhD - Harvard University
Lila Solnica-Krezel, PhD - Washington University School of Medicine
Nadine Vastenhouw, PhD - Max Planck Institute
Participate in this workshop to learn how to best prepare for an interview and gain insight about the interviewing process for a Zebrafish PI position. This webinar will feature an interactive Q&A session with these four leaders.
Online Genome Editing Workshop
July 22, 2020
9:00 - 11:00 a.m. ET
While generating mutations at specific loci has become routine, more sophisticated forms of genome editing in zebrafish remain challenging. Four leaders in this field will be sharing their latest data and advice.
David Grunwald, PhD - University of Utah
Bo Zhang, PhD - Peking University
Filippo Del Bene, PhD - Institut de La Vision
Darius Balciunas, PhD - Temple University
Moderator: Didier Stainier, PhD - Max Planck
Zebrafish Husbandry - Environmental and Husbandry Limits on the Growth of the Zebrafish Model System: What We Need to Improve Upon and How We Do It
June 23, 2020 at 11:00 a.m. and 3:00 p.m. ET
Join expert, Christian Lawrence, in an one-hour webinar that will center around key aspects of zebrafish environmental management and husbandry that are critical to the continued growth and applicability of the model system. Recent scientific and technological advances in nutrition, welfare, population genetics, sanitization, and pathogen control will be highlighted, and their implications for the field will be discussed. An interactive Q&A session will follow the presentation.
Christian Lawrence directs the Aquatic Resources Program (ARP) at Boston Children’s Hospital (BCH). ARP administers the zebrafish program at BCH, which is one of the largest and most active of its kind in the world. Mr. Lawrence also serves as a faculty member for the Health and Colony Management of Laboratory Fish course at the Mount Desert Island Biological Laboratory, and was a Fulbright Specialist at the Bar-Ilan University Faculty of Medicine in Safed, Israel in 2013. He is co-author of The Laboratory Zebrafish, and has written a number of scientific publications on zebrafish biology and culture.
Zebrafish Cardiac Regeneration Workshop
June 15, 2020
10:00 a.m. - 12:30 p.m. ET
Zebrafish have become a very popular model to investigate mechanisms of cardiac regeneration following the pioneering studies by Ken Poss, PhD, in 2002. This webinar will feature presentations by six leaders in this very active field followed by a round table discussion.
Ken Poss, PhD - Duke University Medical Center
Caroline Burns, PhD - Boston Children's Hospital
Kazu Kikuchi, PhD - National Cerebral and Cardiovascular Center
Gilbert Weidinger, PhD - Ulm University
Jeroen Bakkers, PhD - Hubrecht Institute
Nadia Mercader, PhD - University of Bern
Moderator: Didier Stainier, PhD - Max Planck
Grant Writing Basics
May 28, 2020 at 11:00 a.m. and 9:00 p.m. ET
Mary Mullins, PhD
Writing a strong grant proposal requires compelling science, a well written narrative that emphasizes the significance and novelty of your research, and convincing evidence of your ability to accomplish the goals. Get tips on writing a strong, persuasive grant proposal, and avoiding certain pitfalls. Attend this one-hour webinar featuring Mary Mullins, PhD from the University of Pennsylvania. An interactive Q&A session will follow the presentation.
Use of CRISPR/Cas9 for Targeted Integration in Zebrafish
April 29, 2020 at 5:00 p.m. and 9:00 p.m. ET
Jeffrey Essner, PhD
Jeffrey Essner, PhD, from Iowa State University, will present the latest updates on the use of CRISPR/Cas9 for targeted integration in zebrafish.
Dr. Jeffrey Essner is a professor and zebrafish PI at Iowa State University. Over the past several years, he has collaborated with Drs. Maura McGrail, Drena Dobbs, Karl Clark and Stephen Ekker on an NIH R24 project to develop tools for the zebrafish community for targeted integration tools and conditional alleles. His talk will focus on these efforts and highlight the resources developed. A question and answer session will follow the presentation.