Research Technician: John Rawls Lab

Organization: John Rawls Lab
Duke University of Medicine Zebrafish Core Facilities (Z-Core)
Date Posted: 07/06/2021
City: Durham
State: North Carolina
Primary Category: Research
Type of Position: Research Technician II

Description & Details:

Established in 1930, Duke University School of Medicine is the youngest of the nation’s top medical schools. Ranked tenth among its peers, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where traditional barriers are low, interdisciplinary collaboration is embraced, and great ideas accelerate translation of fundamental scientific discoveries to improve human health locally and around the globe.
The Department of Molecular Genetics and Microbiology at Duke University School of Medicine is seeking a research technician with near-immediate availability. The position will be for one year to start with extensions contingent on availability of funding, performance, and needs of the lab.
This position will involve performing a variety of technical and research duties to support the laboratory of Dr. John Rawls ( ) and the Duke University School of Medicine Zebrafish Core Facility (Z-Core)( ). Dr. Rawls’ lab uses zebrafish, mice, and other platforms to investigate mechanisms of host-microbiome interactions in the intestine, and their implications for digestive physiology, nutrition, metabolism, inflammation, and inter-organ communication. Z-Core is a multi-site shared resource that offers aquaculture services to a diverse group of biomedical researchers at Duke University School of Medicine using zebrafish and stickleback as a model organisms.
The successful candidate will have the demonstrated ability to perform, or be willing to learn, the following tasks in a research laboratory: 

  1. Technical duties conducted routinely in a vertebrate animal physiology laboratory such as molecular cloning, DNA and RNA extraction, genotyping, microscopy, imaging, microinjection, animal handling and manipulation. The candidate will be expected to document procedures and collect data in an organized, complete, and accurate fashion.
  2. Laboratory maintenance duties including the preparation of culture media, chemicals, reagents and solutions, as well as assisting with maintaining equipment and lab reagent inventories.
  3. Support duties include assisting faculty, postdocs, students, and staff in the development and execution of new research procedures and techniques. This would include providing support for zebrafish and mouse experiments.

The successful candidate will also have the demonstrated ability to perform, or be willing to learn, the following tasks in a zebrafish aquaculture facility:

  1. Daily zebrafish care and maintenance duties include providing fish feedings, caring for Artemia cultures, checking fish health daily, transferring embryos and providing care to nursery racks.
  2. Daily zebrafish facility maintenance duties include recording water parameters and reviewing for abnormal readings, changing system filters, cleaning, maintenance, and organization of tanks and aquaculture components, assisting facility management in resolving any problems in facility operation, supporting facility sanitation protocols, and assisting in writing and maintaining compliance with IACUC protocols. Occasional weekend hours may be required.
  3. Communicate effectively with of zebrafish facility staff as well as facility users, including explaining and enforcing animal care guidelines and protocols.


Required Qualifications: 
Education/Training: Minimum of a Bachelor’s degree in marine biology, aquaculture, zoology, biology, botany, or other related fields. Experience: Previous laboratory experience is preferred but not required. Demonstrated ability to work effectively in a team and individually is also preferred but not required. Individuals with zebrafish or other aquaculture experience will be given priority, and candidates with relevant experience in rodent or other animal models are also encouraged to apply.

Minimum Qualifications: 
Education: Work generally requires a bachelor's degree in botany, biology, zoology, psychology or other directly related scientific field.
Experience:  None required above education/training requirement. 

To Apply:

To apply for this position, please go here: 
Duke is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, sex, sexual orientation, or veteran status.
Duke aspires to create a community built on collaboration, innovation, creativity, and belonging. Our collective success depends on the robust exchange of ideas—an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes. To achieve this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values.
Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essential job functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.


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