Lab Technician: West Virginia University

Organization:

West Virginia University
Department of Biology - Eberly College of Arts and Science

Date Posted: 11/29/2022
City: Morgantown
State: West Virginia
Primary Category: Research
Type of Position: Lab Tech
 

Description & Details:

West Virginia University Research Corporation is currently accepting applications for a Lab Tech for the Department of Biology in the Eberly College of Arts and Sciences.

The individual to fill this position will perform experiments and gather and analyze data in a developmental neurobiology lab. They will perform overall lab management duties with input and oversight of the lab supervisor (PI) and be responsible for maintaining and managing a small (~400 tank) zebrafish satellite facility that is essential to our research. The individual will help recruit, interview, hire, and train individuals, including students, who work in the lab as well. 

Duties

Zebrafish Facility:

  • Raising and maintaining wild type, mutant, and transgenic zebrafish lines and in vitro fertilization.
  • Shipping and receiving zebrafish lines and laboratory equipment and reagents as needed.
  • Generating mutant and transgenic zebrafish by microinjection of synthesized DNA and RNA, raising multiple generations, and screening.
  • Assuring that the fish facility and laboratory are clean and organized per required safety and operating procedures.
  • Daily zebrafish health and water quality checks, daily feedings of live and dry foods, culturing live foods (rotifers and hatch fry), maintaining detailed fish facility records.
  • Perform fin biopsies of zebrafish for genotyping and line maintenance.
  • Ability to lift and move fish tanks containing up to 6L of water and occasionally transporting lab reagents up to 50 lbs.
  • Respond to and alert supervisor to all fish facility emergencies including those occurring after business hours, on weekends, and on holidays.

General Lab Duties and Experiments:

  • Recommending and implementing with permission improvements to operating conditions or procedures based on evidence and experience. 
  • Stay current on best practices for managing a small university laboratory and zebrafish facility through reading published books and journal articles and attend relevant conferences, workshops, and webinars in these topics.
  • Abiding by, knowing, developing, and teaching all animal care and use practices and lab safety standards of the university and our funding sources.
  • Prepare laboratory and fish facility stock solutions.
  • Ensure lab and fish facility equipment is maintained, operating, and calibrated as needed.
  • Order lab and fish facility supplies as needed and maintain records of all orders.
  • Maintaining excellent experimental records, analyzing data, and reporting to and discussing experimental outcomes and lab and fish. facility management issues with the principal investigator of the lab and more formally at lab meetings.

Personnel Management:

  • Ability to maintain good working relationships with supervisor, other lab members, and faculty and staff in the WVU biology department.
  • Assist lab supervisor in recruiting, evaluating, hiring, and training other lab personnel, including students, in zebrafish care and in standard lab techniques and practices.

Qualifications:

  • Bachelor's in a relevant field (i.e. biology, marine biology, biochemistry, molecular and cellular biology, genetics).  Master's degree preferred.
  • A minimum of one (1) year of experience involving:
    • Research or teaching lab experience.
    • Experience with zebrafish or handling and caring of other aquatic organisms is highly desired. 
    • Basic math and statistics.
    • Introductory chemistry (i.e. lab math for stock solution and other preparations).
  • Knowledge of, willingness to learn and/or experience performing the following laboratory techniques: 
    • DNA, RNA and protein extraction from fish tissue and cells, FACS, PCR, RT-PCR, qPCR, western blotting, molecular cloning, competent cell and agar plate preparation, gel electrophoresis. 
    • Gene/Protein expression analysis: in situ hybridization, immunohistochemistry, RNAseq.
    • Microscopy: light and fluorescence dissecting and compound scopes, confocal imaging, 2-photon imaging.
  • Computer (PC) skills: biological databases and bioinformatics resources, Microsoft Office or equivalent (Excel, Word, Power. Point), PubMed, File Maker Pro or equivalent, other lab database software, image acquisition and processing software.
  • Demonstrated ability to perform biological experiments and analyze data.
  • Demonstrated ability to communicate effectively, ask questions, seek out new knowledge, troubleshoot, and navigate. laboratory issues in an honest, open, and efficient fashion.
  • Demonstrated ability to meet deadlines and manage multiple ongoing tasks successfully at the same time.
  • Demonstrated ability of detailed record keeping.
  • Ability to perform with full professionalism and integrity.

Requirements

  • Must be able to lift between 40-50 lbs (i.e. 5 gallon bucket of water).
  • Must be able to work in a warm (77-78 degree F) and humid (~30-60%) environment.
  • Must be OK handling fish tanks and submerging hands to elbows in water to clean them with PPE.
  • Multitasking and tracking and performing lab duties accurately, correctly, and efficiently is essential, we are a high-pressure research lab environment that needs to generate data for publications and to gain funding; we are subject to many university and federal rules about the work that we perform and have high standards in place for this.

To Apply:

Apply Here

 

 

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