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Workshop: Why Zebrafish Like Light Sheet Microscopy
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When: Saturday, June 23, 2018
Where: Jan Huisken Lab
330 North Orchard Street
Madison, WI  53715
Contact: Susi Powers

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Fluorescence light sheet microscopy (or Selective Plane Illumination Microscopy,SPIM) has pushed the limits of imaging from the in vivo observation of single organs over a few hours towards the observation of whole, developing embryos over the course of several days. The zebrafish, with its rapid, external development andtranslucency, is an ideal model for light sheet microscopy. It has been used to study early development, e.g. gastrulation, as well as organogenesis of cardiac and retinal tissues. The Huisken lab builds customized SPIM across a range of spatial resolutions and sample sizes from single-cell zebrafish embryos to centimeter-sized cleared adult organs. During this workshop, small groups will tour three of our SPIM setups tailored to specific zebrafish imaging. Our first microscope is dedicated to invivo cardiac imaging of the beating heart in 3D across time. Then we demonstratea SPIM designed for imaging large (1-15mm) specimens optically cleared with avariety of clearing protocols. Last, a multi-dire SPIM with custom control and analysis software pipeline for into zebrafish imaging will be shown. This workshop will allow participants to get a better understanding of the strength of sample-tailored SPIM microscopes.

This workshop is limited to 25 registrants.

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